- Targeted LC-MS quantification of key PRMT5 and methionine cycle metabolites — including SDMA, ADMA, SAM, and MTA — to support PRMT5 inhibitor evaluation and oncology research.
- Precision measurement of PRMT5 biomarkers with an MS2-based method that reliably distinguishes SDMA vs. ADMA for clearer insights into PRMT5 activity.
- Validated, high-reproducibility workflow offering a wide dynamic range and strong performance across diverse preclinical and clinical sample types.
Targeted LC-MS Analysis of PRMT5 and Methionine Cycle Metabolites
The Critical Role of PRMT5 Metabolites in Cancer
Protein Arginine Methyltransferase 5 (PRMT5) is a metabolic enzyme frequently upregulated in various cancers, making it a high-value therapeutic target. Panome Bio’s PRMT5 Metabolomics Panel provides insight into PRMT5 and methionine cycle activity for pre-clinical and clinical PRMT5 studies. A targeted metabolomics method is used to profile the primary metabolites involved in this pathway. This service provides robust, relative quantification of key metabolites essential for evaluating the efficacy, selectivity, and response prediction for PRMT5 inhibitors in oncology research and clinical trials.
Accurate measurement of core metabolites is essential for a complete mechanistic fingerprint:
- SDMA (Symmetric Dimethylarginine)
- ADMA (Asymmetric Dimethylarginine)
- MTA (Methylthioadenine)
- SAM (S-adenosylmethionine)
Key Advantages:
- Highly reproducible relative quantification of SDMA, ADMA, SAM, MTA, and related compounds, ideal for comparing treated vs. untreated, or pre- vs. post-treatment samples.
- Specialized MS2-based LC/MS method allows us to reliably distinguish and quantify the isomeric pair of SDMA and ADMA
- Robust measurement across a wide dynamic range for highly reactive metabolites like SAM and MTA by utilizing internal controls.
- Validated method supports a wide range of biological samples, supporting both clinical and preclinical studies.
PRMT5 Panel Metabolites
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- SAM
- MTA
- SDMA
- ADMA
- Methionine
- Homocysteine (HCY)
- S-adenosyl homocysteine (SAH)
- Ornithine
- Acetyl-ornithine
- Acetyl-homoserine
- Adenine
- 5-methylthioribose
- Arginine
- Spermine
- Serine
- Glycine
From Metabolism to Mechanism, Profile PRMT5 Activity in Cancer
Learn how accurately quantifying PRMT5 and methionine cycle metabolites can drive PRMT5 inhibitor development.
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Discover the unparalleled advantages of Panome Bio’s advanced metabolomics technology. Your research project is unique and we can provide a custom experimental design to meet your goals. Whether you are investigating a specific disease model, elucidating biomarkers for diagnostics, or evaluating therapeutic targets, Panome Bio will work with you to design a tailored approach to best suit your research goals.
Ready to elevate your research? Connect with us today and transform your research with metabolomics.